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KMID : 0895820050150010089
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Journal of Oriental Rehabilitation Medicine
2005 Volume.15 No. 1 p.89 ~ p.98
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Effects of Clematidis Radix on Proteoglycan Degradation in Collagenase-induced Rat Osteoarthritis Model
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Lee Dong-Yeul
Kim Soon-Joong
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Abstract
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Objectives: This study was to investigate the effects of Clematidis Radix on Proteoglycan(PG) degradation by measuring of body weight, Glycosaminoglycan(GAG), Interleukin-1β(IL-1β) in synovial fluid, and PG content of articular cartilage of femur in collagenase-induced arthritis in rats.
Methods: Arthritis was induced by injection of collagenase(0.1 ml) into knee joint of rats. Arthritic rats were divided into control(n=8) and treated(n=8) group. Control group was taken normal saline for 15 days and treated group was taken extracts of Clematidis Radix for same duration. Normal group(n=8) was injected with normal saline and was taken normal saline for 15 days. Body weight was measured at 0, 10, 15 days after injection. GAG, IL-1β in synovial fluid were measured with ELISA kit at 15 days after injection. PG content of articular cartilage of femur, represented by safranine O staining, was measured at 15 days after injection. Histopathological study on the articular cartilage of knee joint was investigated at 15 days after injection.
Results: Body weight, PG of treated group, taken Clematidis Radix, were significantly increased, and GAG was significantly decreased compared with control group. But IL-1β was not significantly decreased.
Conclusions: On the basis of these results, we concluded that Clematidis Radix has inhibiting effects on the proteoglycan degradation in collagenase-induced rat osteoarthritis model.
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KEYWORD
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Clematidis Radix, Collagenase-induced osteoarthritis, Proteoglycan, GAG
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